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| Radhika,M.; Saugata,Majumder; Murali,H.S.; Batra,H.V.. |
| Salmonella enterica and Shigella species are commonly associated with food and water borne infections leading to gastrointestinal diseases. The present work was undertaken to develop a sensitive and reliable PCR based detection system for simultaneous detection of Salmonella enterica and Shigella at species level. For this the conserved regions of specific genes namely ipaH1, ipaH, wbgZ, wzy and invA were targeted for detection of Shigella genus, S. flexneri, S. sonnei, S. boydii and Salmonella enterica respectively along with an internal amplification control (IAC). The results showed that twenty Salmonella and eleven Shigella spp., were accurately identified by the assay without showing non-specificity against closely related other Enterobacteriaceae... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Shigella; Multiplex PCR; Spiking. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200041 |
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| Luna Rodríguez, Lorena. |
| De una muestra tomada del rumen de un bovino se aisló una cepa de Shigella sp nov en condiciones anaerobias, y creció en medio selectivo cuya única fuente de carbono fue celulosa, a 39 °C y pH 6.8. La bacteria aislada es Gram negativa, en forma de coco bacilo, con actividad celulolítica cuando se utilizó la tinción con rojo congo en colonias de 10 d. Los resultados filogenéticos indican que se encuentra ubicada en el grupo monofilético perteneciente al género Shigella, pero con 98 % de similitud con respecto a las demás especies del taxa, por lo cual este microorganismo es una especie no reconocida. La utilización de azúcares y alcoholes de la cepa aislada se determinó con el Kit API 50 CH y el Software API WEB. De acuerdo con los resultados hubo... |
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Palavras-chave: Shigella; Rumen; Celulolítica; Monofilético; Cellulolytic; Monophyletic; Ganadería; Doctorado. |
| Ano: 2013 |
URL: http://hdl.handle.net/10521/1860 |
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| Jiménez,Kenia Barrantes; McCoy,Clyde B.; Achí,Rosario. |
| A Multiplex Polymerase Chain Reaction (PCR) assay to be used as an alternative to the conventional culture method in detecting Shigella and enteroinvasive Escherichia coli (EIEC) virulence genes ipaH and ial in lettuce was developed. Efficacy and rapidity of the molecular method were determined as compared to the conventional culture. Lettuce samples were inoculated with different Shigella flexneri concentrations (from 10 CFU/ml to 10(7) CFU/ml). DNA was extracted directly from lettuce after inoculation (direct-PCR) and after an enrichment step (enrichment PCR). Multiplex PCR detection limit was 10(4) CFU/ml, diagnostic sensitivity and specificity were 100% accurate. An internal amplification control (IAC) of 100 bp was used in order to avoid false... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PCR; Shigella; Rapid method; Lettuce; Food. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400018 |
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| Pourakbari,Babak; Mamishi,Setareh; Mashoori,Negar; Mahboobi,Nastaran; Ashtiani,Mohammad H; Afsharpaiman,Shahla; Abedini,Masomeh. |
| Appropriate antimicrobial treatment of shigellosis depends on identifying its changing resistance pattern over time. We evaluated 15,255 stool culture submitted from July 2001 to June 2006 to the Laboratory of Children Medical Center Hospital. Specimen culture, bacterial identification, and disk diffusion susceptibility testing were performed according to National Committee for Clinical Laboratory Standards guidelines. From 15,255 stool samples, 682 (4.5%) were positive for Shigella species. The most common species of Shigella were S. flexneri (48%) and S. sonnei (45%); other results were S. dysenteriae (5%) and S. boydii (2%). The rate of Sensitivity to ceftriaxone (95%), ceftizoxime (94%), and nalidixic acid (84%) were among our isolates. Resistance to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shigella; Antimicrobial susceptibility; Dysentery. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000200007 |
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| Ud-Din,Abu; Wahid,Syeda. |
| Shigellosis produces inflammatory reactions and ulceration on the intestinal epithelium followed by bloody or mucoid diarrhea. It is caused by enteroinvasive E. coli (EIEC) as well as any species of the genus Shigella, namely, S. dysenteriae, S. flexneri, S. boydii, and S. sonnei. This current species designation of Shigella does not specify genetic similarity. Shigella spp. could be easily differentiated from E. coli, but difficulties observed for the EIEC-Shigella differentiation as both show similar biochemical traits and can cause dysentery using the same mode of invasion. Sequencing of multiple housekeeping genes indicates that Shigella has derived on several different occasions via acquisition of the transferable forms of ancestral virulence plasmids... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; E. coli; Shigella; Real-time PCR. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400002 |
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| Barrantes,Kenia; Achí,Rosario. |
| Abstract In Latin America, the disease burden of shigellosis is found to coexist with the rapid and rampant spread of resistance to commonly used antibiotics. The molecular basis of antibiotic resistance lies within genetic elements such as plasmids, transposons, integrons, genomic islands, etc., which are found in the bacterial genome. Integrons are known to acquire, exchange, and express genes within gene cassettes and it is hypothesized that they play a significant role in the transmission of multidrug resistance genes in several Gram-negative bacteria including Shigella. A few studies have described antibiotic resistance genes and integrons among multidrug resistant Shigella isolates found in Latin America. For example, in Brazil, Bolivia, Chile, Costa... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shigella; Antibiotic resistant; Integron; Gene cassettes; Latin America. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400800 |
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